Sequencing and analysis of 51·6 kilobases on the left arm of chromosome XI fromSaccharomyces cerevisiaereveals 23 open reading frames including theFAS1gene

摘要

AbstractWe have sequenced two segments containing a total of 51·6 kb of the left arm from chromosome XI ofSaccharomyces cerevisiae. The first segment of 38·5 kb contains 18 open reading frames (ORFs) of more than 100 amino acid residues. Five ORFs encode known yeast genes, including the fatty acid synthase gene (FAS1). Three new yeast genes were discovered with homologies to non‐yeast genes and ten new genes without homologies to any known sequences. The second segment of 13 kb contains five ORFs with two known yeast genes and three unknown genes. The sequences from cosmid pUKG041 were obtained entirely with the walking primer strategy resulting in a very low overall sequence redundancy of 2·8 and an average reading length of 443 b