Abstract Background Demand for apheresis blood donation has increased with the widening of the use of blood transfusion and the decrease in the donor pool. The knowledge level of apheresis donors, their attitudes such as donating again and recommending others to donate via apheresis are important in meeting this demand. Objective This analytical cross‐sectional study was conducted with 182 plateletpheresis donors to determine their knowledge and attitudes regarding apheresis blood donation. Material and methods Participants were asked 34 questions (which were prepared based on the literature and perfected by expert opinion and pre‐administration) to determine their level of knowledge regarding apheresis. A value of 1 point was assigned for each ‘correct’ answer and 0 points for ‘wrong’ and ‘do not know’ answers. Participants' total level of knowledge scores was formulated to have a value between 0 and 100 (i.e., the score of each group was divided into the number of question and multiplied by 100). Participant attitudes were evaluated based on responses to 14 questions using a 5‐point Likert questionnaire. Results Total knowledge scores regarding apheresis were moderate (55?±?15.2). Those who were educated above the university level (compared to primary school and less, middle and high‐school education levels) had higher level of knowledge scores regarding apheresis. In general, participants had a positive attitude regarding the importance and effects of apheresis blood donation. Those with the following characteristics had a positive attitude (p?
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