Thermal Performance Curves in a Polluted World: Too Cold and Too Hot Temperatures Synergistically Increase Pesticide Toxicity

摘要

Ecotoxicological studies typically cover only a limited part of the natural thermal range of populations and ignore daily temperature fluctuations (DTFs). Therefore, we may miss important stressor interaction patterns and have poor knowledge on how pollutants affect thermal performance curves (TPCs), which is needed to improve insights into the fate of populations to warming in a polluted world. We tested the single and combined effects of pesticide exposure and DTFs on the TPCs of low- and high-latitude populations of Ischnura elegans damselfly larvae. While chlorpyrifos did not have any effect at the intermediate mean temperatures (20-24 °C), it became toxic (reflecting synergisms) at lower (≤16 °C, reduced growth) and especially at higher (≥28 °C, reduced survival and growth) mean temperatures, resulting in more concave-shaped TPCs. Remarkably, these toxicity patterns were largely consistent at both latitudes and hence across a natural thermal gradient. Moreover, DTFs magnified the pesticide-induced survival reductions at 34 °C. The TPC perspective allowed us to identify different toxicity patterns and interaction types (mainly additive vs synergistic) across the thermal gradient. This highlights the importance of using thermal gradients to make more realistic predictions about the impact of pesticides in a warming world and of warming in a polluted world.