The hypothesis that the regulative action of the barley embryo on amylase synthesis in the aleurone layer is partially exerted by a mechanism of osmotic regulation was tested.When embryo growth was retarded by a drought treatment no concomitant repression of amylase production could be detected. Inhibition of growth by a cold treatment was not accompanied by an elevation of osmotic pressure or by a depression of the amylase level in the endosperm. Osmotic concentration of de-embryonated seeds was lower than that of the endosperm of intact seedlings.We concluded that the results do not support the tested hypothesis.
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