Facile synthesis of biogenic silica nanomaterial loaded transparent tragacanth gum hydrogels with improved physicochemical properties and inherent anti-bacterial activityf

摘要

In this report,biogenic,crystalline(-60.5 ± 2)bowknot structured silica nanoparticles(BSNPs)of length ~ 274 ± 7 nm and width ~ 36 ± 2 nm were isolated from invasive species viz.Lantana camara.These were then chemically modified using nitrogen containing moieties viz.APTES and CTAB.These modified BSNPs were then used as electrostatic cross-linking agents for the formation of tragacanth gum(TG)hydrogels.The cytocompatible CTAB@BSNP-TG hydrogels documented-10-12 fold enhancement in anti-bacterial activity against Staphylococcus aureus and Pseudomonas aeruginosa when compared with TG hydrogels.Disruption of the bacterial membrane by ROS generation and protein leakage were responsible for anti-bacterial activity.A cell migration assay suggested that CTAB@BSNP-TG augmented the cell proliferation of NIH-3T3 cells compared to other TG hydrogels.The present study will pave the path for the development of organic-inorganic hybrid nanocomposite-based hydrogels for anti-bacterial and cell migration applications.