Application of Advanced Proteomic and Mass Spectrometry Technologies to Enhance the Efficacy of Production of Biopharmaceuticals

摘要

The Chinese Hamster Ovary (CHO) cell line is the dominant mammalian expression system for biopharmaceutical production. Improving the efficiency of production of these biologics will be critical in controlling costs to healthcare systems as more of these drugs come to market. Proteomic and mass spectrometry analysis has been used to profile recombinant CHO cells to get a greater understanding of the molecular and cellular mechanisms that result in a high producing cellular phenotype. In this review we will focus on the application of mass spectrometry (MS) to characterize the proteome of CHO cells and also to delve deeper into the proteome by discussing how mass spectrometry analysis of cellular post translational modifications could potentially play a key role in understanding transcription, growth, cellular signalling events, protein degradation and apoptosis in recombinant CHO cells. Such events are likely to be key to understanding and controlling bioprocess relevant phenotypes to improve the efficacy of production of high quality biotherapeutic proteins.