Determination of the number of Epstein—Barr virus genomes in whole blood and red cell concentrates

摘要

SUMMARY.The risk of Epstein—Barr virus (EBV) infection after blood transfusion has been controversially discussed. Little is known about EBV transmission via buffy‐coat‐depleted red cell concentrates (RCC). In this study, we determined the number of EBV genomes in RCC of EBV‐seropositive donors in comparison to whole blood. RCC were prepared from whole blood donations by using the ‘top and bottom system’. Leucocyte content was significantly reduced in RCC in comparison to whole blood (0.47 times 109vs. 2.3 times 109per unit;P<0.001). As B cells are expected to harbour EBV genomes, we analysed the number of B lymphocytes in both types of blood products. There was a significant reduction of B cell content from a median value of 90 times 106in whole blood to 0.2 times 106in RCCs (P<0.001). The number of EBV genomes was estimated at a median value of seven from 106B cells in the peripheral blood of healthy, EBV‐seropositive blood donors by means of a polymerase chain reaction (PCR) assay. By calculation, one unit of RCC may contain an average of one to two EBV genomes, in contrast to a whole blood unit, which is likely to harbour an average of 600 to 700 EBV genomes. It is concluded that the use of leucocyte depletion systems significantly reduces the number of EBV genomes in erythrocyte concentrates. Thus, leucocyte reduced blood products appear to minimize the risk of