Dual-Wavelength Comparative Imaging Based on a Novel NIR-II/IIb Emissive Rare Earth- Doped Nanoparticle

摘要

The second near-infrared biological window two (NIR-II, 1000–1700 nm)imaging has made remarkable achievements in fluorescence imaging ofdiseases. Here, this work takes advantage of the rich spectral properties ofrare-earth doped nanoparticles (RENPs) and their capability to presentmultiple emission peaks in different luminescence regions simultaneously;novel RENPs are synthesized that have emission peaks in the NIR-II1000nm(1000–1300 nm) region and enhanced emission in the NIR-IIb(1500–1700 nm) window and are named as NEU-RENPs. These RENPs withnew compositions enable a fair comparison to evaluate their imagingperformance in NIR-II_(1000 nm) and NIR-IIb regions under the same conditions.The imaging abilities of the resulted nanoparticles in NIR-II_(1000 nm) and NIR-IIbregions are investigated systematically for local blood vessels, tumor vessels,acute inflammatory vessels, arterial thrombosis, cerebrovascularinflammation, and brain injury models. The results indicate that imaging ofNIR-IIb shows higher resolution and sensitivity than that of NIR-II_(1000 nm), andit has a stronger ability to accurately distinguish normal and pathologicaltissues. Interestingly, the fuzzy image of NIR-II_(1000 nm) reveals moreinformation about the background structure of the target, which is difficult tobe achieved in NIR-IIb imaging. The results prove NEU-RENPs based NIR-Iibimaging is preferable for biomedical applications.