H_2S-stimulated bioenergetics in chicken erythrocytes and the underlying mechanism

摘要

Jin Z, Zhang Q, Wondimu E, Verma R, Fu M, Shuang T, Arif HM, Wu L, Wang R. H_2S-stimulated bioenergetics in chicken erythrocytes and the underlying mechanism. Am J Physiol Regul Integr Comp Physiol 319: R69-R78, 2020. First published May 20, 2020; doi:10.1152/ajpregu.00348.2019.—The production of H_2S and its effect on bioenergetics in mammalian cells may be evolutionarily preserved. Erythrocytes of birds, but not those of mammals, have a nucleus and mitochondria. In the present study, we report the endogenous production of H_2S in chicken erythrocytes, which was mainly catalyzed by 3-mercaptopyruvate sulfur transferase (MST). ATP content of erythrocytes was increased by MST-generated endogenous H_2S under normoxic, but not hypoxic, conditions. NaHS, a H_2S salt, increased ATP content under normoxic, but not hypoxic, conditions. ATP contents in the absence or presence of NaHS were eliminated by different inhibitors for mitochondrial electron transport chain in chicken erythrocytes. Succinate and glutamine, but not glucose, increased ATP content. NaHS treatment similarly increased ATP content in the presence of glucose, glutamine, or succinate, respectively. Furthermore, the expression and activity of sulfide:quinone oxi-doreductase were enhanced by NaHS. The structural integrity of chicken erythrocytes was largely maintained during 2-wk NaHS treatment in vitro, whereas most of the erythrocytes without NaHS treatment were lysed. In conclusion, H_2S may regulate cellular bioenergetics as well as cell survival of chicken erythrocytes, in which the functionality of the electron transport chain is involved. H_2S may have different regulatory roles and mechanisms in bioenergetics of mammalian and bird cells.