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机译:Rational Regulation of Reaction Specificity of Nitrilase for Efficient Biosynthesis of 2-Chloronicotinic Acid through a Single Site Mutation
Zhejiang Univ Technol;
Nitrilase; reaction specificity; hydrolysis activity; hydration activity; 2-chloronicotinic acid; protein engineering; PSEUDOMONAS-FLUORESCENS EBC191; ENANTIOSELECTIVE PRODUCTION; (S)-MANDELIC ACID; HYDRATASE; ARYLACETONITRILASE; EVOLUTION; IDENTIFICATION; MUTAGENESIS; HYDROLYSIS; VARIANTS;