White light-emitting, biocompatible, water-soluble metallic magnesium nanoclusters for bioimaging applications

摘要

Ultra-small (1.6 nm), water-soluble, white light-emitting (WLE), highly stable (similar to 8 months) BSA templated metallic (Mg-0) nanoclusters (fluorescent magnesium nanoclusters = FMNCs) is developed using the green and facile route. Synthesis was facilitated by the reduction of magnesium salt, where template bovine serum albumin is utilized as a reducing agent and ascorbic acid act as a capping agent to impart stability in water, thereby obtaining stabilized Mg-0 nanoclusters In solution, stabilized Mg-0 nanoclusters produce white light (450-620 nm with FWHM similar to 120 nm) upon 366 nm light excitation. This white light emission was found to have a CIE coordinate of 0.30, 0.33 [pure white light CIE (0.33, 0.33)]. Taking advantage of WLE and ultrasmall size, FMNCs were used for in vitro fluorescence imaging of HaCaT cell lines, yielding blue (tau = 2.94 ns, with a relative of QY = 1.2 % w.r.t QS), green (tau = 3.07 ns; relative quantum yield of 4.6% w.r.t R6G) and red (tau = 0.3 ns) images. Further, incubation of FMNCs with HEK293 (Human embryonic kidney cell) and cancerous MDA-MB-231 (Breast cancer cell line) human cell lines yielded 100 % cell viability. Current work is envisioned to contribute significantly in the area of science, engineering, and nanomedicine.