Characterization of kinetic, thermodynamic, and binding properties of L-phenylalanine molecuiarly imprinted polymer

摘要

L-Phenylalanine imprinted polymeric adsorbent was fabricated by a non-covalent approach utilizing methacrylic acid as functional monomer, ethylene glycol dimethacrylate as cross-linker, acetonitrile as porogen, and acetic acid and trifluoro-acetic acid as additives (template:monomer:cross-linker ratio was 1:5:24). Adsorption equilibrium, kinetic, thermodynamic, and enantioselective studies were carried out characterization of adsorbent. Parallel studies were performed for the binding of enantiomers on non-imprinted polymer. The experimental L- and D-phenylalanine adsorption-binding isotherms were fitted with hybrid Langmuir-Freundlich model. Studies have demonstrated a heterogeneous distribution of binding sites, site with high and low binding affinity, in polymer. The thermodynamic test revealed the entropy controlled adsorption process on L-phenylalanine imprinted adsorbent. Kinetic studies indicated second-order rate adsorption process. The adsorbent is characterized by enantioselectivity for recognition of phenylalanine enantiomers.