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EFFECTS OF HEAVY METAL ON Tetraselmis chuii

机译:重金属对四蒲苜蓿的影响

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摘要

We determined the toxic effects of seven metals, copper(Cu), manganese (Mn),zinc(Zn),cobalt(Co), molybdate(Mo),lead(Pb),andcadmium(Cd) on the microalgae Tetraselmis chuii (Butcher,1959). We exposed 50 mL of cultivated microalgae(f/2 Guil-lard) in the exponential growth phase, with three rep-licates, to concentrations of0(control),0.1,1.0,5.0, 10,25,and 50ugL-1ofeach metal for 72 hours. The cellular count was recorded daily to evaluate any po-tential lethal effects. An increase in cellular density was seen in the control treatment. All treatments ex-posed to metals showed a logarithmic increase in cell density within 48 hours, with a cellular decrease that became evident later on. This study result with an in-significant decrease at low concentrations of Cd, Pb, and Cu at 24 hour was probably attributed to their use as micronutrients at these low concentrations. The most lethal effect was observed with Cd treat-ment, which killed 50 of the microalgae population at a concentration of 0.62 μg L-1In this study, this concentration was three times lower than that oflead and mercury, while being ten times lower than that of cobalt and molybdate. To elucidate the adverse ef-fects on seawater environments, the microalgae Tetraselmis chui is recommended as amodel species.
机译:测定了铜(Cu)、锰(Mn)、锌(Zn)、钴(Co)、钼酸盐(Mo)、铅(Pb)和镉(Cd)7种金属对微藻的毒性作用(Butcher,1959)。将50 mL培养的微藻(f/2 Guil-lard)暴露在指数生长期,含有3种代表物,浓度为0(对照)、0.1、1.0、5.0、10、25和50ugL-1,持续72 h。每天记录细胞计数以评估任何潜在的致死作用。在对照治疗中观察到细胞密度的增加。所有暴露于金属的处理都显示出细胞密度在48小时内的对数增加,细胞减少后来变得明显。这项研究结果显示,在24小时内,低浓度的Cd、Pb和Cu的含量下降不显著,这可能归因于它们在这些低浓度下用作微量营养素。镉处理观察到最致命的效果,在0.62μgL-1的浓度下杀死了50%的微藻种群在这项研究中,该浓度比铅和汞低三倍,而比钴和钼酸盐低十倍。为了阐明对海水环境的不利影响,建议将微藻 Tetraselmis chui 作为模型物种。

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