A Sensitive Double Isotope Modification of the Farr Assay Using Beta-Particle Emitters

摘要

A sensitive modification of the ammonium sulfate precipitation assay for antibody affinity is described. The assay combines small reaction volumes and a32P volume marker to determine the average relative affinity of small amounts of antibody to DNP hapten. The assay is relatively safe since two beta-particle emitters are utilized instead of two gamma-ray emitters. The assay has proven useful for the determination of small amounts of antibody such as might be found in secretions and column fractions.