Comparative Study of Different Predictive Methods of Peptides Retention Time on Chromatographic Reversed-Phase Columns

摘要

Two peptides samples were studied in order to compare the validity of some predictive retention times. We examined the predictive retention times stated by Guo and based on the relative hydrophobic contribution of each amino acid residue, by Mant who takes into account the length of the peptides and by Chabanet who discriminates three groups of amino acids. Although Chabanet's model provided good results, we found that a more general correlation was obtained with Mant's retention prediction especially in the case of peptides containing a high fraction of basic residues.