Universal Calibration in Aqueous Size Exclusion Chromatography with On-Line Differential Viscometry Using Commercial TSK-PW Columns

摘要

Poly(ethylene oxide), poly(ethylene glycol), and poly(saccharide) exhibit universal calibration under aqueous SEC conditions with commercial TSK-PW columns using on-line, differential viscometry. The product of log(intrinsic viscosity molecular weight) versus retention volume converges to a single curve for these polymers. Mobile-phase compositions of 0.1 N sodium nitrate and 20% aqueous acetonitrile at 35° C are used to examine this behavior. Mark-Houwink constants for these polymers are determined directly from on-line specific viscosity measurements.