Differential antigen recognition by T cell populations from strains of mice developing polar forms of granulomatous inflammation in response to eggs ofSchistosoma mansoni

摘要

AbstractIn humans, infection with schistosome helminths can lead to dissimilar forms of clinical disease. Likewise, in the experimental mouse system, identical infection protocols withSchistosoma mansonicause a more severe granulomatous disease in the C3H strain than in the C57BL/6 strain. To address this difference, we developed panels of schistosomal egg antigen (SEA)‐specific T cell hybridomas to compare the responses of C3H and C57BL/6 mice to the major egg antigen p40. All derived C3H T cell hybridomas, despite being clonally distinct and restricted by either I‐Akor I‐Ek, responded to recombinant fragment 15‐1 of the p40 antigen, while none of the C57BL/6 T cell hybridomas did. Consistent with the observed monoclonal T cell responses, polyclonal lymph node cells from schistosome‐infected C3H mice reacted strongly to fragment 15‐1, which contrasted sharply with the weak response displayed by the C57BL/6 strain. Moreover, studies with congenic mice demonstrated that the strong CD4+T cell response to fragment 15‐1 was under major histocompatibility complex control and segregated with the H‐2khaplotype. These findings suggest that a dominant T cell response against a major egg antigen may represent a risk factor for the development of