Bile Acids. Lxx. Preparative Separation of Kryptogenin from Companion Sapogenins by High Performance Liquid Chromatography

摘要

Commercial samples of kryptogenin or its acetate can be purified by preparative high performance liquid chromatography on a PrepPAK-500/Silica cartridge. The free alcohol is separated from accompanying sapogenin with a mixture of chloroform:methanol (50:1), whereas the acetates are separated well with a mixture of methylene chloride:hexane (2:3). The companion sapogenins, diosgenin and yamogenin, 25R- and 25S- isomers, were separated by analytical HPLC with hexane-isopropanol (100:1) or as the acetates with hexane:isopropanol (250:1). Characterization of kryptogenin and yamogenin was completed with1H-NMR, IR and MS spectrometry.