Lymphocyte cultures from the gorilla, chimpanzee, and orangutan were treated with the oligopeptide antibiotic distamycin A. This AT-specific DNA-ligand induces a distinct undercondensation in the quinacrine-bright heterochromatin of the gorilla and chimpanzee. This is also the case in human lymphocyte cultures. Distamycin A further causes an undercondensation in the nonheterochromatic bands 17q21 of the gorilla and 16q22 of man. No visible distamycin A-sensitive chromosome regions were determined in the orangutan. The in vitro treatment with distamycin A preserves the somatic pairings between the quinacrine-bright heterochromatic regions existing in the interphase nucleus until the succeeding metaphase stage. The phylogenetic origin of the quinacrine-bright and distamycin A-sensitive heterochromatin in the ancestor of man, the gorilla, and the chimpanzee is discussed.
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机译:大猩猩、黑猩猩和猩猩的淋巴细胞培养物用寡肽抗生素二他霉素 A 处理。这种 AT 特异性 DNA 配体在大猩猩和黑猩猩的奎纳克林明亮异染色质中诱导明显的凝聚不足。在人类淋巴细胞培养物中也是如此。双霉素 A 进一步导致大猩猩的非异色带 17q21 和人类的 16q22 冷凝不足。在猩猩中没有发现可见的双霉素 A 敏感染色体区域。用双霉素A进行体外治疗保留了存在于间期核中的奎纳克林-明亮异色区域之间的体细胞配对,直到随后的中期阶段。讨论了人类、大猩猩和黑猩猩祖先中奎纳克林-明亮和双霉素 A 敏感异染色质的系统发育起源。
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