In situ observation of adsorption process of hemoglobin on quartz surfaces by slab optical waveguide spectroscopy

摘要

In situ observation of the adsorption phenomena of hemoglobin on both hydrophilic and hydrophobic quartz surfaces has been performed by slab optical waveguide spectroscopy. The absorption intensity of hemoglobin increased for more than one hour on hydrophobic surfaces, while it took only a few minutes to reach the equilibrium value in the case of hydrophilic ones. The adsorbed hemoglobin remained stable on quartz surfaces even after changing of solutions in the cell, and showed the activities of reduction and oxidation by Na{sub}2S{sub}2O{sub}4 solution and O{sub}2 in air, respectively.