Thymus gland fractionation and thymocyte lysis

摘要

AbstractMethods are described for the isolation of two separate fractions from guinea pig thymus gland; i.e., Hassall's corpuscles and stromal segments. Hassall's corpuscles are relatively dense epithelial aggregates sedimentable at low centrifugal acceleration, while the lighter stromal segments remain in the supernatant fluid. Solubilization of the thymocytes, effected by high‐speed homogenization in hypertonic glycerol, is unaccompanied by visible gelation or nuclear residue. Glycerol‐sensitivity resembles radiation‐sensitivity in many res