AbstractHistology, histochemistry, and ultrastructure of the Harderian gland and lacrimal gland of the lizardPodarcis s. siculawere investigated. The Harderian gland, located at the medial corner of the orbit, can be divided into three zones showing different tinctorial features either with Mallory or hematoxylineosin stains. The glandular cells of the acinar medial zone secrete predominantly acidic sulphated mucosubstances. The acinar cells of the intermediate zone contain secretory granules that show a weak reaction to the histochemical tests for mucosubstances. The lateral zone has a tubulo‐acinar type of structure and tests strongly for proteins, whereas Alcian‐PAS staining is very weak. The lacrimal gland is smaller than the Harderian gland and lies in the region of the posterior commissure of the eyelids. It shows the same histological and histochemical characteristics of the medial zone of the Harderian gland, i.e., it is mucous secreting. At the ultrastructural level the zonation is well defined, especially when the secretory granules are examined. Granules of the mucoid type are found in the lacrimal gland and the medial zone of the Harderian gland. The secretory granules of the lateral part of the Harderian gland show a composite structure never described before. Therefore, they have been called “special secretory granules”. Each of these granules is composed of three sharply separated components. It is not known whether the three components correspond to different secretions. Histochemical tests suggest that they are of the serous type. Both mucous and serous granules are secreted by the same glandular cells of the intermediate zone of the Harderian gland. The two types of granules usually occupy different cell compartments. The mechanism of secretion appears either merocrine or apocrine in both the Harderian gland and the lacrima
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