Effect of Alkyl Chain Length of Bonded Silica Phases on Separation, Resolution and Efficiency in High Performance Liquid Chromatography

摘要

A comparative study of alkyl bonded phases was carried out under optimum solvent conditions for each phase. Three columns, RP-2, RP-8 and RP-18, were tested for their efficiency and resolving power using three groups of compounds in three binary organic-water mobile phases. The organic solvents were acetonitrile, methanol and tetrahydrofuran which are widely used as solvent modifiers.