Solid Phase Reactors as an Analytical Tool in the Determination of Urinary Noradrenaline and Adrenaline

摘要

The possibility of performing the oxidation of the catecholamines noradrenaline and adrenaline inside a PbO2- or a MnO2- solid phase reactor (SPR), which is integrated in the chemical reaction detector of the trihydroxyindole (THI) method is demonstrated. The adaptation of the SPR to the chromatographic conditions and the optimization of the different reaction parameters is described. A comparison of the two SPRs to the ferricyanide oxidation in the homogenous system is made with respect to accuracy, sensitivity and precision of the results. The new SPR-technique enables simplification and low cost running of the THI-system in routine analysis of urinary catecholamines.