AbstractCH strain chickens selected from the F2 progeny of a cross between CH (B10/10) and B14B (B14/14) strains camed bursa (BA) and thymus (TA)‐specific alloantigens of B14B parental origin. The BA marker was present on 95 % of bursal cells but only on 10 % of splenic and blood B lymphocytes. The TA marker was expressed by 80 % of thymus cells and weakly by 45 % of splenic and 70 % of blood T lymphocytes. The adopted immunolo‐gical and genetic protocol offers a feasible approach towards detection of differentiation antigens in major histocompatibility complex‐homozygous but ‘minor’ alloantigen‐segregating
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