Effective renaturation of met~(-1) lysozyme expressed in Escherichia coli as inclusion bodies

摘要

The effective renaturation method that we developed was applied to the renaturation of the reduced Met~(-1) lysozyme expressed in E. coli as inclusion bodies. Using a slow dialysis renaturation method, a concentration as high as 1.25 mg/ml of the reduced Met~(-1) lysozyme could be renatured in 40% yield, while the folding yield was less than 5% even at a concentration of 0.25 mg/ml using a rapid dilution method.