Anion-dependent changes of energy transfer in chloroplasts III. Evidence for inactivation of light-triggered adenosine triphosphatase in chloroplasts by chloride

摘要

Washing chloroplasts with a high concentration of Tris-Cl-buffer caused Cl-dependent inhibition of photophosphorylation, light-induced pH rise and light-triggered Mg2+-dependent ATPase activity. The inhibition of these activities was largely prevented by the presence of 10−4MADP or ATP during Tris washing, especially that of Mg2+-ATPase activity. The results were interpreted as suggesting that the inactivation of light-triggered ATPase activity in chloroplasts by chloride is one of the causes of the uncoupling of chloroplasts with Tris washin