Chemical Studies on Tobacco Smoke LXVII. Quantitative Determination of Alkaloids in Tobacco by Liquid Chromatography

摘要

An HPLC method was developed for the quantitative determination of individual alkaloids of the basic fraction of tobacco extracts. Two reverse phase RP-18 columns were used, eluting with a gradient of aqueous triethylamine phosphate buffer (pH 7.56) and acetonitrile.