Recombinant human interleukin 5 is an eosinophil differentiation factor but has no activity in standard human B cell growth factor assays

摘要

AbstractFollowing the observation that mouse interleukin 5 (IL 5) is active as a B cell growth factor (BCGF) as well as an eosinophil differentiation factor, this work was carried out to test recombinant human IL 5 for BCGF activity. A highly active, partially purified batch of recombinant human IL 5 was prepared and tested for BCGF activity in four laboratories. This batch gave a 50% endpoint of 1:77450 in the human eosinophil differentiation assay, 1:983 in the mouse eosinophil differentiation assay and 1:42 in the mouse BCL1assay, thus demonstrating that, like mouse IL 5, human IL 5 has cross‐species activity. By comparison with the assays in the mouse this batch would be expected to have 50% maximal human BCGF activity of about 1:4000. In each assay a known positive factor was used as a positive control, and there was no inhibitory activity in the preparation. However, despite the activity towards the mouse B cell lymphoma, the results showed no detectable activity in a panel of assays used to identify human BCGF and B cell differentiation factors. These assays included (a) proliferation assays with tonsillar or splenic B cells in the presence of the co‐stimulators anti‐μ or phorbol myristate acetate; (b) a restimulation assay in which tonsillar B cells are first activated with eitherStaphylococcus aureusCowan 1 or a mixture of phorbol dibutyrate and ionomycin, or splenic B cells are first activated with anti‐μ; (c) production of immunoglobulin by B cells in a restimulation assay withStaphylococcus aureusCowan 1; (d) production of immunoglobulin by the Epstein‐Barr virus‐transformed B lymphoblastoid CESS cell line; (e) the ability to stimulate proliferation of chronic lymphocytic leukemia (B‐CLL) cells freshly explanted from three different patients; (f) the ability to stimulate the B lymphoma (L4) cell line and the mature B cell (HBF1) line, and (g) the ability to replace T cells in specific antibody responses.It therefore seems unlikely that recombinant human IL 5 is either a growth or a differentiation factor for human B cells, and raises the interesting question of the biological significance of the BCGF activity of this fact