Effects of Cytokinin and Anticytokinin on the Initial Stage of Adventitious Bud Differentiation in the Epidermis ofToreniaStem Segments

摘要

In the epidermis ofToreniastem segments cultured in vitro, meristematic zones (MZ) were initiated prior to adventitious bud differentiation. Application of benzyladenine (BA) stimulated MZ and bud formation, and the average number of MZ per epidermal strip increased linearly with rising concentrations of BA. The presence of naphthaleneacetic acid together with BA in the medium suppressed MZ formation. Some derivatives of 4-substituted-2-methylpyrrolo[2,3-d]pyrimidine which have anticytokinin activity inhibited BA-promoted MZ formation. Interactions of various cytokinins and anticytokinins in MZ and adventitious bud formation were also examined. The number of MZ formed by treatment with 5μM BA was reduced 50%by the simultaneous application of one of the anticytokinins at the same concentration