AbstractDensities of the various bone fractions i.e., water, organic, volatile inorganic and ash fractions, as well as fat in the various animals have been studied. However. values reported by various workers failed to show consistency and uniformity, especially with respect to the volatile inorganic (often called “CO2fraction”) and the ash fractions. These densities are prerequisite to the study of the volumetric composition of bone and bone marrow.In our study, the fat and organic densities found were similar to those previously reported. The volatile and non‐volatile inorganic (ash) fractions, had density values of 1.684 and 3.180, respectively. The ash density was much higher than values previously reported but it verifies a theoretical value deduced by the authors from published work.Our higher ash density was due to a smaller measured volume of this fraction obtained by applying a negative pressure to remove the small entrapped air bubbles from the ash sample prior to the water displacement measurement. Because a smaller portion of the total inorganic volume was used for the ash calculation, giving it a higher density, a larger balance of the total inorganic volume was left for the volatile inorganic density calculation. This accounted for ash and volatile inorganic densities which were higher and lower, respectively, than such values previously reported.Prior to the determination of the density of the non‐volatile inorganic fraction, the ashing time and temperature were studied. Curves plotted from the results of such studies were described and ex
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