Owing to the presence of free living amoeba – some ofwhich are pathogenic to man– in any aqueous medium (before or after traditional treatment processes in surface water, groundwater. swimming pools, etc.). our task wasto test the efficiency of ozone asa disinfecting agent. We began by studying the ozonation process, in bactericidal and virucidal conditions, on cysts of eleven free living amoeba strains. Most of thetested strains were destroyed under less drastic conditions than those arising from true ozonation (0.4 mg/L of dissolved ozone residual maintained for 4 minutes) and we attempted to find the ozonation parameters (dissolved ozone residual and contact time) Just necessary to obtain inactivation of the cystsunder laboratory conditions.
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