A Method of Measuring Dipalmitoylphosphatidylcholine (DPPC) by High‐Performance Liquid Chromatography (HPLC): Effect of Dexamethasone on DPPC Secretion in Fetal Rat Alveolar Type II Cell Culture*

摘要

AbstractObjectives: To establish a method by which to measure dipalmitoylphosphati‐dylcholine (DPPC), which is the most prevalent phospholipid in lung surfactant, by high‐performance liquid chromatography (HPLC), and to measure DPPC secretion in type‐II alveolar cell culture to determine whether glucocorticoid has a direct accerelating effect.Method: Type‐II alveolar cell suspensions were made with fetal rat lungs and then cultured. Dexamethasone (10‐9, 10‐8M) was added to some of the culture dishes. DPPC was extracted from the culture medium, purified, and measured by high‐performance liquid chromatography (HPLC).Results: In the measurements of DPPC, the specificity of isolation and measurement parity were excellent coefficient of variation: intraassay; 5.66 (0.005 mg/ ml), 25.37 (0.05 mg/ml)): interassay; 8.35 (0.005 mg/ml)), 0.08 (0.05 mg/ml)). The DPPC concentration in the culture dishes of the dexamethasone was not significantly different than that of the control dishes (Student'st)‐test).Conclusion: The above results prove that dexamethasone does not directly stimulate lung alveola