Optimization of cultural conditions for the production of L-DOPA (L-3, 4 dihydroxy phenyl alanine) from L-Tyrosine by Aspergillus oryzae

摘要

The present study is concerned with the optimization of different cultural conditions lot tile production of L-DOPA from L-Tyrosine by surface culture technique. Of the 12 different isolates of Aspergillus oryzae tested, for production of mycelium, strain ISB-9 was found to give maximum yield of L-DOPA (0.34 mg/ml). L-DOPA production was maximum at pH 3.0 of the reaction mixture, 50 deg C, 50 minutes and 2.5 mg/ml, optimum temperature, time and L-Tyrosine concentration, respectively.