Blood Donations Indeterminate in HIV‐1 Western Blot Analysed by IgM Immunoblot and Polymerase Chain Reaction

摘要

AbstractThe presence of IgM antibodies to human immunodeficiency virus 1 (HIV‐1) was investigated in blood donor sera which were indeterminate in anti‐HIV‐1 IgG Western blot testing. In 7 of 173 instances out of approximately 1,000,000 blood donation sera with an isolated anti‐p24 IgG produced an anti‐gp41‐45 IgM immunoblot reaction. Applying polymerase chain reaction (PCR) to 29 indeterminate samples out of approximately 125,000 blood donations it was found that 2 of them were IgM‐positive and also contained HIV‐1‐specific DNA sequences. Eleven months later 1 of these 2 donors was retested and found IgM