Determination of Acetylcholine and Choline in Human Plasma Using High-Performance Liquid Chromatography Combined with an Immobilized Enzyme Reactor

摘要

A method for assaying Acetylcholine (ACh) and Chloine (Ch) simultaneously in human plasma by high-performance liquid chromatography with electrochemical detection (LCEC) combined with an immobilized enzyme reactor is described for the first time in this paper. Human plasma samples were obtained from 12 volunteers free of neurological and psychological disease. The volunteers were from 22 to 41 years old (mean 30.1 years), eight being males. Extraction of ACh and Ch from plasma was performed according to the liquid cation-exchange method. Concentrations of ACh ranged from 57.0 to 179.6 pmol/ml (mean and S.D. of 121.6 and 40.9 pmol/ml respectively). The present method, LCEC combined with an immobilized enzyme reactor is raped, simple and sensitive compared with the biological methods, GC-MS and RIA for analyzing ACh and Ch in plasma.