Remarks on the Calibration of Steric Exclusion Liquid Chromatography by Polymolecular Standards

摘要

The relation of polymer molecular characteristics versus chromatographic parameters in steric exlusion liquid chromatography (GPC) was considered as a typical black box problem. Practical problems were discussed for cases of the GPC black box calibration with polymolecular polystyrene standards and of using an improper calibration model. It was concluded that checking the reliability of the GPC black box calibration is of utmost importance especially when narrow calibration standards are not available.