Immunochemical Difference of Wound-Induced 1-Aminocyclopropane-1-carboxylate Synthase from the Auxin-Induced Enzyme

摘要

Immunochemical cross-reactivity of wound- and auxin-induced 1-aminocyclopropane-1-carboxylate (ACC) synthase was examined with the antibody against wound-induced ACC synthase purified from mesocarp of winter squash (Cucurbita maximaDuch.). The antibody recognized ACC synthase from wounded hypocotyls of winter squash and from wounded pericarp of tomato fruits, but not the enzyme from IAA-treated hypocotyls of winter squash, tomato and mung bean. These results indicate that the primary structure of the wound-induced enzyme is different from that of the auxin-induced enzyme in the same species, and implies that there are two different genes for ACC synthase, one for wound induction and the other for auxin induction.