T cell proliferation induced by anti‐cd3 antibodies: requirement for a t‐t cell interaction

摘要

AbstractIn the presence of interleukin 2 (IL2), soluble anti‐CD3 monoclonal antibodies can stimulate highly purified normal T lymphocytes to proliferate. In these experiments HLADR+T cells constituted 13 to 20% of the total cell population, and other HLADR−cells, such as monocytes and B lymphocytes, constituted less than 1% of the population. When the HLADR+T cells were removed from the total T cell population by cytofluorometric sorting, the residual HLADR−T cells failed to respond to soluble anti‐CD3 plus IL2. When the separated HLADR+T cells were recombined with the HLADR−T cells, a response was again found. This response was dependent on the dose of HLADR−T cells added in the mixture. Irradiation individually of the HLA DR+and DR−T cells revealed that the proliferation in the cell mixture was predominantly, if not exclusively, by the HLADR−T cells. The ability of the HLA DR+T cells to provide a signal necessary to this proliferation was radioresistant. These data indicate that under the conditions of these experiments HLADR+(“activated”) T cells provide a signal necessary to the responsiveness of previou