Nitric oxide and oxygen radicals induced apoptosis via bcl-2 and p53 pathway in hypoxia-reoxygenated cardiomyocytes

摘要

Neonatal rat cardiomyocytes were subjected to 24 h of hypoxia 95%N_2/5%CO_2 and 24 h of hypoxia plus 4 h of reoxygenation 95%O_2/5%CO_2. 24 h of hypoxia increased the levels of NO, NO_2~-/NO_3~-, TBARS and LDH. 24 h of hypoxia plus 4 h of reoxygenation decreased the levels of NO, NO_2~-/NO_3~-, but further increased TBARS and LDH. The hypoxia up-regulated the expression of bcl-2, p53 and p21/waf1/cip1 but the reoxygenation down-regulated the expression of bcl-2, and further up-regulated p53 and p21/waf1/cip1. The hypoxia increased cell apoptosis and reoxygenation further increased both apoptotic and necrotic cell death. NO, NO_2/NO_3~-, TBARS, DNA frag- mentation and cell apoptosis were enhanced by SNP and inhibited by L-NAME respectively. In addition, SOD/catalase down-regulated the expression of p53, p21/wafl/cip1 and TBARS but up-regulated bcl-2 and increased indirectly the level of NO, NO_2~-/NO_3~-, and inhibited DNA frag- mentation. The results suggest that hypoxia-induced cell death is associated with the activation of NO, bcl-2 and p53 pathway, while hypoxia-reoxygenation induced cell death via the generation of reactive oxygen species and activation of p53 pathway. The present study clarified that NO may be an initiative signal to apoptotic cell death and the activation of bcl-2, p53 and p21/waf1/cip1 pathway in hypoxic and hypoxia-reoxygenated cardiomyocytes.