Thy‐1 positive tingible body macrophages (TBM) in mouse lymph nodes

摘要

AbstractThis study was prompted by the observation that cells in mouse lymph nodes (LN) with cytological characteristics of tingible body macrophages (TBM) appeared to be Thy‐1 positive. The objective of this study was to determine if these large cells were TBM and to conclusively demonstrate their reactivity for Thy‐1. The cells were studied using monoclonal antibodies (MoAb) against Thy‐1 and macrophage markers including F4/80 and Ia antigens at both light microscopic (LM) and electron microscopic (EM) levels. Immunocytochemical reactivity by TBM for Thy‐1 antigen specific MoAb was demonstrated by LM in both in situ and in vitro LN preparations. Furthermore, ultrastructural examination of these germinal center cells in situ demonstrated that the Thy‐1 reactivity visualized at the LM level was associated with ribosomes and endoplasmic reticulum as well as their plasma membrane. Similarly, these cells expressed intracytoplasmic and membrane reactivity for Ia antigens and also for the macrophage specific antigen F4/80. This indicates that the reactivity is due to active synthesis of the Thy‐1 antigen and not attributable to reactivity of any phagocytosed Thy‐1 positive cells. As defined by their germinal center location and morphological characteristics, these Thy‐1 reactive macrophages were identified as TBM. Germinal center TBM thus represent a unique, vigorously phagocytic subset of mature macrophages which express both macrophage and th