Euglena graciliswas grown under controlled levels of iron such that chlorophyll synthesis was halted, although the cells continued to grow at a normal rate. Chloroplasts isolated by zonal centrifugation from iron-deficient cells, even at a very early stage of deficiency, contained less than half the normal levels of RNA and ribosomes. Total RNA was unaffected. We note that a defect in chloroplast ribosomes could account for the signs of iron deficiency chlorosis.
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