The use of radioactively labelled riboprobes for in situ hybridizaiotn; background and examples of applicaiton

摘要

Abstract:An overivew is provided concerning the preparation and use of radiolabelled single‐stranded RNA probes (ribooprobes) forin situhybridization. All theoretical aspects are covered as weel as some practical examples from the authors' experience. The use of riboprobes forin situhybridizaiton represents a valid and highly sensitive alternative to oligonucleotide probes. A sampel protocol is finally described in detail: this should enable any investigators to design the ISH assay that best fits their particular need