A simple and practical method for the determination of virginiamycin (VGM) in premixes and feeds is described. VGM in premixes and feeds is extracted with methanol, and subsequently analysed by high performance liquid chromatography (HPLC) with ultraviolet detector set a wavelength at 235 nm. The column is Kaseisorb LC 0DS-300-5 and the eluent is acetonitrile-water (35:65, v/v). The VGM concentration in various samples is calculated from the peak hight of virginiamycin-M1(M1), of main component of VGM. Amounts of 0.2–20.0 μg/ml of VGM can be determined. The detection limit for VGM is 2.0 ng. The average recoveries were 93.3% and 86.6% for VGM added to feeds at the level of 20 μg/g and 10μg/g, respectively.
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