A region-specific microdissection library for human chromosome 2p23→p21 and the analysis of an interstitial deletion of 2p21

摘要

A region-specific library of human chromosome 2p23→p21 was constructed using microdissection and micro-cloning techniques. Analysis of 94 single-copy microclones from the library showed that 64% were derived from the dissected region. Ten microclones were further mapped to the 2p21 region using a patient with an interstitial deletion of 2p21 and displaying holoprosencephaly, an abnormal embryonic development in midbrain and midfac