Studies on the immune response of the Numigall, a hybrid of domestic cock and guinea hen

摘要

AbstractImmunization of the Numigall with sheep red blood cells or dinitrophenylated, bovine γ‐globulin induced an immune response, similar to that of both parental species in terms of circulating antibodies and rosette‐forming cells.The main class of normal 7 S immunoglobulins of the three avian species was found to be identical with regard to electrophoretic migration, gel filtration and amount of125I fixed by both heavy and light chains. The results are compatible with a four chain molecule consisting of two heavy (68 000 daltons) and two light (20 000 daltons) chains.The avian molecules migrated faster in electrophoresis and came out earlier in gel filtration than human IgG. The molecular weight difference was mainly due to the heavy chains.A rabbit anti‐human IgG serum demonstrated antigenic cross‐reactions with the main class of avian 7 S immunoglobulins at the level of their separated heavy and light chains.The cross‐reactions were mainly located in the Fab fragment and very little cross‐reactivity could be attributed to the Fc region of