Fabrication and evaluation of the all transparent micro-PCR chip

摘要

A microchannel chip for quantitative continuous-flow polymerase chain reaction (PCR) was developed. The microchannel was fabricated on a cover glass substrate using standard photolithography and wet-etching techniques, and sealed by another cover glass substrate. Indium-tin-oxide (ITO) thin films served as heaters and temperature sensors were integrated on the chip. A microscope and ICCD camera system was used to detect the accumulation of double-stranded DNA (dsDNA) in-situ using SYBR Green I dye. Finally, a 97-bp segment of the mouse X gene from mouse tongue cDNA was PCR-amplified with the chip.