FISH analysis of micronuclei in uncultured human T lymphocytes provides a convenient new possibility to assess structural and numerical chromosome damage in vivo. In women, T-cell micronuclei mostly contained whole chromosomes (71.6 ), especially the X chromosome (28.5). Cell culture (72 h) enhanced the frequency of micronuclei harboring sacentric fragments 2.9 fold and the X chromosome 1.5 fold. X-chromosome-positive micronuclei were particularly prevalent (42.0) in binucleate cells produced by cytochalasin B, a cytokinesis inhibitor used to identify cells that have divided in vitro. This was explained by a decrease in autosome-containing and an increase in X-positive micronuclei.
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机译：对未培养的人T淋巴细胞中的微核进行FISH分析，为评估体内结构和染色体损伤提供了一种方便的新可能性。在女性中，T细胞微核大多包含全染色体（71.6%），尤其是X染色体（28.5%）。细胞培养（72 小时）使携带中心片段的微核频率提高了 2.9 倍，将 X 染色体提高了 1.5 倍。X染色体阳性微核在细胞松弛素B（一种用于识别体外分裂细胞的胞质分裂抑制剂）产生的双核细胞中特别普遍（42.0%）。这可以通过常染色体含量的减少和X阳性微核的增加来解释。
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