Circulating and skeletal muscle microRNA profiles are more sensitive to sustained aerobic exercise than energy balance in males

摘要

Abstract MicroRNAs (miRNAs) regulate molecular processes governing muscle metabolism. Physical activity and energy balance influence both muscle anabolism and substrate metabolism, but whether circulating and skeletal muscle miRNAs mediate those effects remains unknown. This study assessed the impact of sustained physical activity with participants in energy balance (BAL) or deficit (DEF) on circulating and skeletal muscle miRNAs. Using a randomized cross‐over design, 10 recreational active healthy males (mean?±?SD, 22?±?5 years, 87?±?11?kg) completed 72?h of high aerobic exercise‐induced energy expenditures in BAL (689?±?852?kcal/day) or DEF (?2047?±?920?kcal/day). Blood and muscle samples were collected under rested/fasted conditions before (PRE) and immediately after 120?min load carriage exercise bout at the end (POST) of the 72?h. Trials were separated by 7?days. Circulating and skeletal muscle miRNAs were measured using microarray RT‐qPCR. Independent of energy status, 36 circulating miRNAs decreased (P?