AbstractInDrosophila melanogasterthe yolk protein (YP) genes are normally expressed only in the fat body and follicular epithelium of adult females‐never in males or in larvae. We describe here a first step toward a genetic examination of the developmental controls that restrict the activity of the YP genes to adult female tissues. A YP1 promoter that contains the tissue‐, temporal‐, and sex‐specific controlling elements for expression was fused to the reporter gene, alcohol dehy‐drogenase (Adh). The gene fusion was transformed into an Adh‐deficient genotype. As assayed by a number of criteria, that the fusion gene is expressed in the same physiological manner as the endogenous yolk protein genes. The fusion gene's activity is modulated intransby a temperature‐sensitive allele of the sex determination gene,tra‐2.The Adh enzyme serves as a selectable marker and therefore these flies are suitable for use in genetic screens for trons‐acting mutations that affect the expression of the y
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